Composite

Part:BBa_K1634008:Design

Designed by: Xinhong Chen   Group: iGEM15_China_Tongji   (2015-09-14)


pmyo2-dsRed ( C.elegans )


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 288
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1178
    Illegal SapI.rc site found at 198


Design Notes

Since we use the restriction enzyme site to do the ligation, we should pay attention to the sequence of Pmyo2 and dsRed, avoiding using the restriction site exits in it.


Source

We get Pmyo2 from the C.elegans' genomic DNA by PCR. DsRed is a commonly used reporter,so we get it from some plamids by PCR. Then we ligated the Pmyo2 into PPD95.75, a vector commonly used in C.elegans. Finally, we ligated the dsRed into PPD95.75_Pmyo2.


References